Gene Insertion in Caenorhabditis elegans via Novel DNA Insertion Technique
by Alyssa J. Hudnall
Faculty advisor: Dr. James Hampton
Previous methods used to insert genetic information into the genome of the nematode, Caenorhabditis elegans, have experienced numerous complications; therefore, we have begun to experiment with a new method. Also, selectable markers for gene insertion analysis are rare in these nematodes. The purpose of our research is to insert various genes that could possibly be utilized as a selectable marker in C.elegans using an Aerosol Beam Injector.
A definitive protocol has yet to be determined for this novel technique. This method is much like gold-particle bombardment; however, an aerosolized form of DNA is used as a vector instead of gold-particles. Multiple parameters will be varied in these tests to determine the optimal settings for the machine as well as the preparation of the samples.